Journal: Frontiers in Microbiology

Article Title: Genomic analysis, culturing optimization, and characterization of Escherichia bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce

doi: 10.3389/fmicb.2024.1486333

Figure Lengend Snippet: FIGURE 4 Detection of PCR gene products of Shiga toxins (stxl, stx2), intimin (eaeA), and β glucuronidase (uidA) in Escherichia coli O157:H7 EDL933 and E. coli O157:H7 B6-914, which were used in phage propagation and enumeration, and in Escherichia bacteriophage OSYSP (phage OSYSP) grown in these two host strains. (A) Host strains. (B) Host strains and phage OSYSP.

Article Snippet: Strain Inhibition area by spot test Plaque formation during titer determination Escherichia coli O157:H7 EDL933 +++ +++ E. coli O157:H7 B6-914 +++ +++ E. coli O157:H7 Sakai +++ +++ E. coli K12 +++ +++ E. coli O157 E5 − − Salmonella Senftenberg − − Salmonella Saintpaul − − Salmonella Enteritidis − − Salmonella Kentucky − − Salmonella Typhimurium ATCC BAA-185 − − Salmonella Tennessee + ++ Salmonella Poona − − Salmonella Typhimurium LT2 +++ − Salmonella Montevideo ++ − Salmonella Javiana ++ − Salmonella Eimsbuettel − − Salmonella Typhimurium DT105 − − +++ Clear plaques or complete inhibition zones.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Genomic analysis, culturing optimization, and characterization of Escherichia bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce

doi: 10.3389/fmicb.2024.1486333

Figure Lengend Snippet: FIGURE 6 Changes in the populations of Escherichia bacteriophage OSYSP (phage OSYSP) and the host, Escherichia coli O157:H7 EDL933 during one-step growth curve experiments. (A) Phage OSYSP titers (log10 PFU/mL). (B) Turbidity changes, measured as OD600nm, due to changes in the population E. coli O157:H7 EDL933; corresponding population count changes are shown in Table 4. Each data point represents the average from three independent trials. Error bars indicate ± standard deviations.

Article Snippet: Strain Inhibition area by spot test Plaque formation during titer determination Escherichia coli O157:H7 EDL933 +++ +++ E. coli O157:H7 B6-914 +++ +++ E. coli O157:H7 Sakai +++ +++ E. coli K12 +++ +++ E. coli O157 E5 − − Salmonella Senftenberg − − Salmonella Saintpaul − − Salmonella Enteritidis − − Salmonella Kentucky − − Salmonella Typhimurium ATCC BAA-185 − − Salmonella Tennessee + ++ Salmonella Poona − − Salmonella Typhimurium LT2 +++ − Salmonella Montevideo ++ − Salmonella Javiana ++ − Salmonella Eimsbuettel − − Salmonella Typhimurium DT105 − − +++ Clear plaques or complete inhibition zones.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Genomic analysis, culturing optimization, and characterization of Escherichia bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce

doi: 10.3389/fmicb.2024.1486333

Figure Lengend Snippet: FIGURE 7 Effect of different Escherichia bacteriophage OSYSP titers on clear zone formation when spotting on a lawn of (A) Escherichia coli O157:H7 B6-914, and (B) Salmonella Typhimurium LT2. Concentration of Escherichia bacteriophage OSYSP stock suspension was 109 PFU/mL; 10 μL of phage stock and its decimal dilutions were spotted, which corresponds to a dilution of 100 (spotted on the upper right side of the plates) and subsequent dilutions, clock-wise, were 10–1 through 10–4.

Article Snippet: Strain Inhibition area by spot test Plaque formation during titer determination Escherichia coli O157:H7 EDL933 +++ +++ E. coli O157:H7 B6-914 +++ +++ E. coli O157:H7 Sakai +++ +++ E. coli K12 +++ +++ E. coli O157 E5 − − Salmonella Senftenberg − − Salmonella Saintpaul − − Salmonella Enteritidis − − Salmonella Kentucky − − Salmonella Typhimurium ATCC BAA-185 − − Salmonella Tennessee + ++ Salmonella Poona − − Salmonella Typhimurium LT2 +++ − Salmonella Montevideo ++ − Salmonella Javiana ++ − Salmonella Eimsbuettel − − Salmonella Typhimurium DT105 − − +++ Clear plaques or complete inhibition zones.

Techniques: Concentration Assay, Suspension

Journal: Microbiology

Article Title: The interaction of Escherichia coli O157 :H7 and Salmonella Typhimurium flagella with host cell membranes and cytoskeletal components

doi: 10.1099/mic.0.000959

Figure Lengend Snippet: Strains used or constructed in this study

Article Snippet: TUV93-0 , E.coli O157:H7; EDL933 (ATCC 700927) stx − , [ ] .

Techniques: Construct, Transduction, Mutagenesis

Journal: International Journal of Nanomedicine

Article Title: Development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria

doi: 10.2147/ijn.s132784

Figure Lengend Snippet: Figure 3 Effects of nano-dispersions on biofilm formation. The effects of different concentrations of (A) CNMA, (B) Si-GNPs and (C) CNMA-GNPs, on biofilm formation by E. coli O157:H7, P. aeruginosa, MSSA 6538, and MRSA were examined after incubation for 24 h in 96-well plates without shaking. At least three independent experiments were conducted. (D) Representative 3D projection confocal laser scanning microscopy images of E. coli O157:H7, P. aeruginosa, MSSA 6538, and MRSA biofilms after treatment with free CNMA, Si-GNPs, and CNMA-GNPs or none (control). *P,0.05 versus the control. Abbreviations: CNMA, cinnamaldehyde; GNP, gold nanoparticle; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-sensitive Staphylococcus aureus; Si, silica.

Article Snippet: Bacterial strains and growth conditions The bacterial strains used were enterohemorrhagic Escherichia coli (E. coli) O157:H7 [ATCC 43895, EDL933], Pseudomonas aeruginosa (P. aeruginosa PAO1), methicillinsensitive Staphylococcus aureus (MSSA 6538) [ATCC 6538], and methicillin-resistant Staphylococcus aureus (MRSA) strain [ATCC BAA-1707].

Techniques: Incubation, Confocal Laser Scanning Microscopy, Control

Journal: International Journal of Nanomedicine

Article Title: Development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria

doi: 10.2147/ijn.s132784

Figure Lengend Snippet: Figure 5 Kaplan–Meier survival curves for nematodes infected with S. aureus. Results of killing assays of C. elegans strain fer-15; fem-1 infected with S. aureus and fed with Si-GNPs or CNMAGNPs. Non-treated infected nematodes were considered as none. The percentage survivals shown represent the results of three independent experiments (n=60) performed. E. coli OP50 was the common food source and used as the control strain. Abbreviations: CNMA, cinnamaldehyde; GNP, gold nanoparticle; Si, silica.

Article Snippet: Bacterial strains and growth conditions The bacterial strains used were enterohemorrhagic Escherichia coli (E. coli) O157:H7 [ATCC 43895, EDL933], Pseudomonas aeruginosa (P. aeruginosa PAO1), methicillinsensitive Staphylococcus aureus (MSSA 6538) [ATCC 6538], and methicillin-resistant Staphylococcus aureus (MRSA) strain [ATCC BAA-1707].

Techniques: Infection, Control